This prospective study was approved by the Institutional Animal Care and Use Committee. Fifteen male 129S1 mice were used. At the age of 10 weeks all mice underwent a baseline MRI (K.J. and C.M.F., 6 and 3 years of experience in small-animal MRI, respectively), after which they were selected for RAS (n=10) or sham (n=5) surgeries (see Appendix ) by an experienced technologist (H.T., 24 years of experience). To longitudinally monitor progression of fibrosis, follow-up MRI scans were performed (K.J. and C.M.F.) 2, 4 and 6 weeks post-surgery, intervals shown to induce progressive renal injury (25 (link)). Animals were weighed and blood pressure measured (H.T.) after MRI by tail-cuff (XBP1000 system, Kent Scientific, Torrington, CT).
Protocol detail
Longitudinal Renal Fibrosis Progression
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Animals
Blood pressure
Fibrosis
Injury
Institutional animal care and use committee
Male
Mice
Mri scans
Progression
Renal
Surgery
Tail
Corresponding Organization :
Other organizations : Mayo Clinic
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Variable analysis
independent variables
- RAS surgery
- Sham surgery
- Progression of fibrosis
- Animal weight
- Blood pressure
- Age of mice (10 weeks)
- Strain of mice (129S1)
- Gender of mice (male)
- Experience of MRI technicians (K.J. - 6 years, C.M.F. - 3 years)
- Experience of surgery technologist (H.T. - 24 years)
- Sham surgery as a negative control
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