Immunofluorescence and Western Blotting Antibodies

The following antibodies were used for immunofluorescence. rabbit anti- γH2AX (ab11174, Abcam); mouse anti-γH2AX (ab26350, Abcam); rabbit anti-c-Kit (3074S, Cell Signaling Technology); rabbit anti-GFRα1; (AF560-SP, R and D Systems); rabbit anti-Ki67 (ab15580, Abcam); rabbit anti-MPC1 (HPA045119, Millipore Sigma); mouse anti-MTCOI (ab14705, Abcam); mouse anti-Ndufb6 (ab110244, Abcam); rabbit anti-PLZF (SC-22831, Santa Cruz Biotech); mouse anti-PLZF (SC-28319, Santa Cruz Biotech); rabbit anti-SCP3; (ab15093, Abcam); mouse anti-SCP3 (ab97672, Abcam); mouse anti-SDHA (ab14715, Abcam); guinea pig anti-SP-10 (Gift from Prabhakara P. Reddi); rabbit anti-Tom20 (SC-11415, Santa Cruz, Biotech); mouse anti-Total OXPHOS (ab110413, Abcam). The following antibodies were used for western blotting. Chicken anti-Mfn1 (Chen et al., 2003 (link)); rabbit anti-Mfn2 (D2D10S #9482, Cell Signaling Technology); mouse anti-tubulin (T6199, Sigma).

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Varuzhanyan G., Rojansky R., Sweredoski M.J., Graham R.L., Hess S., Ladinsky M.S, & Chan D.C. (2019). Mitochondrial fusion is required for spermatogonial differentiation and meiosis. eLife, 8, e51601.

Publication 2019

ab11174 ab26350 ab15580 ab14705 ab110244 SC-22831 SC-28319 ab15093 ab97672 ab14715 SC-11415 ab110413 T6199

Antibodies Chicken Guinea pig Immunofluorescence Mfn2 Mouse Rabbit Tubulin

Corresponding Organization :

Other organizations : California Institute of Technology

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Variable analysis

independent variables

Antibody type (e.g., rabbit anti-γH2AX, mouse anti-γH2AX, rabbit anti-c-Kit, rabbit anti-GFRα1, rabbit anti-Ki67, rabbit anti-MPC1, mouse anti-MTCOI, mouse anti-Ndufb6, rabbit anti-PLZF, mouse anti-PLZF, rabbit anti-SCP3, mouse anti-SCP3, mouse anti-SDHA, guinea pig anti-SP-10, rabbit anti-Tom20, mouse anti-Total OXPHOS, chicken anti-Mfn1, rabbit anti-Mfn2, mouse anti-tubulin)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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