Soluble OX40 and sOX40L were measured in serum from patients with AD and from HC with a sOX40 ELISA kit (cat. no. BMS296TEN, eBioscience (eBioscience, San Diego, CA, USA)) and with an inhouse sOX40L ELISA, as described previously (7 (link), 17 (link)). OD values were read by a Thermo Scientific Multiscan GO reader (Thermo Scientific, Waltham, MA, USA) at 450 nm, with 570 nm as a reference. The minimum detectable level of sOX40 and sOX40L was determined by the cut-off value of the standard curve. The lowest detectable levels of sOX40 and sOX40L were 1.9 and 0.039 ng/ml, respectively.
Protocol detail
Measurement of soluble OX40 and OX40L
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Corresponding Organization : Aarhus University Hospital
Other organizations : Agilent Technologies (Denmark), Kiel University, Aarhus University
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Variable analysis
independent variables
- Soluble OX40 (sOX40)
- Soluble OX40L (sOX40L)
- Serum levels of sOX40
- Serum levels of sOX40L
- Patients with atopic dermatitis (AD)
- Healthy controls (HC)
- Positive control: Not specified
- Negative control: Not specified
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