CD8+T cells were separated from PBMCs of HLA-A2 healthy donor by EasySep™ Human CD8+T Cell Isolation Kit (Stemcell, Canada).24 (link) CD8+T cells were co-cultured with peptide-loaded DCs (CD8+T cells to DC ratio was 10:1). rhIL-2 (20 ng/mL) was added on the 2nd day, and the culture medium was changed in half every 2–3 days. On days 7 and 12 of culture, the same proportion of peptide-loaded DCs was added for enhanced stimulation.26 (link),27 (link) Cultured to day 15, cells were collected as effector T cells (i.e., CTLs).
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Expansion of CD8+ Cytotoxic T Cells
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Corresponding Organization : Guangxi University of Chinese Medicine
Other organizations : The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine
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Variable analysis
independent variables
- Isolation of CD8+ T cells from PBMCs of HLA-A2 healthy donor using EasySep™ Human CD8+ T Cell Isolation Kit
- Co-culture of CD8+ T cells with peptide-loaded DCs (CD8+ T cells to DC ratio was 10:1)
- Addition of rhIL-2 (20 ng/mL) on the 2nd day of culture
- Addition of the same proportion of peptide-loaded DCs on days 7 and 12 of culture for enhanced stimulation
- Cultured CD8+ T cells on day 15 as effector T cells (i.e., CTLs)
- Healthy donor used for CD8+ T cell isolation
- Changing culture medium in half every 2–3 days
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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