An expanded Materials and Methods section is available in the online data supplement at http://circres.ahajournals.org and describes the following: inducible Nkx2-5 knockout mice; Southern, Northern, and Western blotting and immunostaining; surface ECG recording and ultrasound imaging; histological analyses; gene expression profiling; measurement of sodium current and simultaneous recording of cardiac contraction and Ca2+; real-time RT-PCR; reporter assays; and statistical analyses.
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Cardiac Transcription Factor Regulation
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Corresponding Organization :
Other organizations : Tokyo Medical and Dental University, University of California, San Francisco, Boston Medical Center, University Medical Center, Boston University, Yamaguchi University, Massachusetts General Hospital, Washington University in St. Louis, Nagoya University, Florida College, University of Florida
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Protocol cited in 9 other protocols
Variable analysis
independent variables
- Inducible Nkx2-5 knockout mice
- Surface ECG recording
- Ultrasound imaging
- Histological analyses
- Gene expression profiling
- Measurement of sodium current
- Simultaneous recording of cardiac contraction and Ca2+
- Real-time RT-PCR
- Reporter assays
- Southern blotting
- Northern blotting
- Western blotting
- Immunostaining
- Statistical analyses
- Positive controls not explicitly mentioned.
- Negative controls not explicitly mentioned.
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