Accurate Animal Body Composition Analysis

Gold-standard methods for determining BC of animals were established over 50 years ago and involve killing the mouse²⁰ (Supplementary Note 2). For longitudinal measures, there are various options such as isotope dilution, dual-energy X-ray absoptiometry, total body electrical conductivity, magnetic resonance spectroscopy, magnetic resonance imaging and computed tomography (Supplementary Note 2). Generally it is good practice to use both destructive and non-destructive methods as they provide complementary information, including individual organ and fat depot weights as well as measures of fat cell size and number. When high accuracy is required, destructive methods of BC analysis are the methods of choice. Magnetic resonance spectroscopy and dual-energy X-ray absoptiometry systems provide the next best alternatives, in that order. Total body electrical conductivity and isotope-dilution methods are relatively poor. All indirect methods should be calibrated against destructive methods to yield the best results.

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Tschöp M.H., Speakman J.R., Arch J.R., Auwerx J., Brüning J.C., Chan L., Eckel R.H., Farese RV J.r., Galgani J.E., Hambly C., Herman M.A., Horvath T.L., Kahn B.B., Kozma S.C., Maratos-Flier E., Müller T.D., Münzberg H., Pfluger P.T., Plum L., Reitman M.L., Rahmouni K., Shulman G.I., Thomas G., Kahn C.R, & Ravussin E. (2011). A guide to analysis of mouse energy metabolism. Nature methods, 9(1), 57-63.

Publication 2011

Animals Body Computed tomography Dilution Electrical conductivity Gold Isotope Isotope dilution methods Magnetic resonance spectroscopy X ray

Corresponding Organization :

Other organizations : Technical University of Munich, Chinese Academy of Sciences, University of Aberdeen, Institute of Genetics and Developmental Biology, University of Buckingham, École Polytechnique Fédérale de Lausanne, Max Planck Society, Max Planck Institute for Metabolism Research, Baylor College of Medicine, University of Colorado Denver, University of California, San Francisco, University of Chile, Beth Israel Deaconess Medical Center, Yale University, University of Cincinnati Medical Center, Pennington Biomedical Research Center, Columbia University, National Institutes of Health, University of Iowa, Joslin Diabetes Center, Harvard University

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Variable analysis

independent variables

Destructive methods of body composition (BC) analysis
Non-destructive methods of BC analysis (isotope dilution, dual-energy X-ray absoptiometry, total body electrical conductivity, magnetic resonance spectroscopy, magnetic resonance imaging and computed tomography)

dependent variables

Measures of body composition, including individual organ and fat depot weights, as well as measures of fat cell size and number

control variables

Not explicitly mentioned

controls

Destructive methods of BC analysis (considered the gold-standard and method of choice when high accuracy is required)
Non-destructive methods of BC analysis (calibrated against destructive methods to yield the best results)

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