The P. mirabilis strains (KP; 5628) were isolated at the Department of Microbiology from the urine of patients of the Children’s Memorial Health Institute in Warsaw, Poland, who had been diagnosed with infectious urolithiasis. The P. mirabilis K8/MC strain was obtained from urinary stones and provided by the Provincial Specialist Hospital M. Pirogow in Lodz. The strains were identified using the API 20E test (Biomerieux, Marcy-I’Etoile, France) and cultured on TSB (tryptic soy broth, BTL, Warsaw, Poland) at 37 °C for 24 h.
Lactobacillus strains were isolated from the urinary tract of healthy people with the consent of the Committee for Bioethics of Scientific Research of the University of Lodz (4(I)/KBBN-UŁ/II/2020), and were deposited in the bacterial strain collection at the Department of Biology of Bacteria, University of Lodz. The method of isolation of these strains and their characteristics had been described previously [17 (link)]. Briefly, the strains were obtained from human urine of both men and women who had not been treated with antibiotics and probiotics in the last 3 months. They were identified by their morphology and mass spectrometry using a MALDI/TOF Microflex LT (Bruke, Billerica, MA, USA). Lactobacillus spp. were cultured on APT broth (BD Difco, Franklin Lakes, NJ, USA) and incubated in 5% CO2 at 37 °C for 48 h.
Lactobacillus strains were isolated from the urinary tract of healthy people with the consent of the Committee for Bioethics of Scientific Research of the University of Lodz (4(I)/KBBN-UŁ/II/2020), and were deposited in the bacterial strain collection at the Department of Biology of Bacteria, University of Lodz. The method of isolation of these strains and their characteristics had been described previously [17 (link)]. Briefly, the strains were obtained from human urine of both men and women who had not been treated with antibiotics and probiotics in the last 3 months. They were identified by their morphology and mass spectrometry using a MALDI/TOF Microflex LT (Bruke, Billerica, MA, USA). Lactobacillus spp. were cultured on APT broth (BD Difco, Franklin Lakes, NJ, USA) and incubated in 5% CO2 at 37 °C for 48 h.
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