Human HNSCC lines CAL-33, CAL-27, SCC-9, and SCC-25 were obtained from ATCC and maintained in the Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) + GlutaMAX™ media supplemented with 10% heat inactivated fetal bovine serum (FBS) and 100 U/ml penicillin streptomycin. Mouse oral squamous cell carcinoma models, MOC1-esc1 and MOC2, were maintained in IMDM/Hams-F12 (2:1) supplemented with 5% heat inactivated FBS, 100 U/ml penicillin streptomycin, 5 ng/ml EGF (Millipore), 400 ng/ml hydrocortisone (Sigma Aldrich), and 5 μg/ml insulin (Sigma Aldrich). Cell lines were routinely tested for mycoplasma and underwent short tandem repeat (STR) cell line authentication at the DFCI within 6 months of use. MOC1-esc1, an anti-PD-1 resistant line, is isogenic to the previously described MOC1 model (27 (link)). Derivation and further characterization will be described elsewhere (Zhou et al., in preparation). C57BL/6 mice (6-week-old, females) were from Taconic and OT-1 mice were purchased from Jackson Labs (C57BL/6-Tg (TcraTcrb)1100Mjb).
Protocol detail
Cell Lines for HNSCC and Oral Cancer Research
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C57bl mice
Cell line authentication
Cell lines
Eagle
Females
Fetal bovine serum
Hams
Hnscc
Human
Hydrocortisone
Insulin
Mice
Mycoplasma
Nutrient
Oral squamous cell carcinoma
Penicillin
Short tandem repeat
Streptomycin
Corresponding Organization : Dana-Farber Brigham Cancer Center
Other organizations : Hefei University of Technology
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Variable analysis
independent variables
- Cell lines used: CAL-33, CAL-27, SCC-9, SCC-25 (human HNSCC lines), MOC1-esc1 and MOC2 (mouse oral squamous cell carcinoma models)
- No specific dependent variables mentioned
- Cell culture media: Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) + GlutaMAX™ media supplemented with 10% heat inactivated fetal bovine serum (FBS) and 100 U/ml penicillin streptomycin for human HNSCC lines
- Cell culture media: IMDM/Hams-F12 (2:1) supplemented with 5% heat inactivated FBS, 100 U/ml penicillin streptomycin, 5 ng/ml EGF, 400 ng/ml hydrocortisone, and 5 μg/ml insulin for mouse oral squamous cell carcinoma models
- Cell lines were routinely tested for mycoplasma and underwent short tandem repeat (STR) cell line authentication at the DFCI within 6 months of use
- Mouse models: C57BL/6 mice (6-week-old, females) and OT-1 mice
- Positive control: None mentioned
- Negative control: None mentioned
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