Protein was extracted from liver tissue or cell cultures as described [19 (link)]. The NICD, Bax, Bcl-2, c-caspase-3, TRAF6, p-TAK1 (Ser412), TAK1, p-P65 (Ser536), P65, RIPK3, p-MLKL (Ser345), Lamin B2, β-actin (Cell Signaling Technology) were used. The Western ECL substrate mixture (Bio-Rad) was used to image with the iBright FL1000 (ThermoFisher Scientific). See Additional file 1: Materials.
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