Two derivatives of green fluorescent protein-labeled 4T1 (CRL-2539; ATCC) cells were kindly provided by Professor Jonas Fuxe (Karolinska Institute, Solna, Sweden). Lentivirus-mediated knockdown of C/EBPβ was described previously in Johansson et al.4 (link) Sh C/EBPβ cell line expressed C/EBPβ shRNA, whereas sh control expressed control shRNA. Both cell lines were cultured in RPMI-1640 (Gibco, Waltham, MA, USA) supplemented with 10% fetal calf serum and 1% penicillin and streptomycin in a humidified incubator at 37 °C in an atmosphere containing 5% CO2 and 95% O2.
Protocol detail
Lentiviral Knockdown of C/EBPβ in 4T1 Cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Atmosphere
Cell lines
Cells
Derivatives
Fetal calf serum
Green fluorescent protein
Lentivirus
Penicillin
Shrna
Streptomycin
Corresponding Organization :
Other organizations : Karolinska Institutet, Postgraduate School of Molecular Medicine, Medical University of Warsaw, Lund University, Skåne University Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Lentivirus-mediated knockdown of C/EBPβ
- Not explicitly mentioned
- Culture medium (RPMI-1640 supplemented with 10% fetal calf serum and 1% penicillin and streptomycin)
- Incubation conditions (37 °C, 5% CO2, 95% O2, humidified)
- Sh control cell line expressed control shRNA
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!