Protocol detail

Quantifying Selenium in Bacterial Cultures

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Se-enriched culture media and bacteria were collected at the beginning and at the end of the 48-h growth period. After harvesting, the bacterial pellets were washed, weighed and lysed via sonication. The Se content in the samples was measured in duplicates by total reflection X-ray fluorescence (TXRF) spectrometry, using a bench-top TXRF spectrometer (S2 Picofox, Bruker Nano, Berlin, Germany) with 1 mg/L Yttrium (Merck/Millipore; Darmstadt, Germany) as internal standard, as described [32 (link),33 (link)].

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Zytner P., Kutschbach A., Gong W., Ohse V.A., Taudte L., Kipp A.P., Klotz L.O., Priebs J, & Steinbrenner H. (2024). Selenium-Enriched E. coli Bacteria Mitigate the Age-Associated Degeneration of Cholinergic Neurons in C. elegans. Antioxidants, 13(4), 492.

Publication 2024

S2 Picofox Yttrium

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Variable analysis

independent variables

Growth period duration (48-h)

dependent variables

Selenium (Se) content in bacterial samples

control variables

Bacterial culture media (Se-enriched)
Yttrium (1 mg/L) as internal standard for TXRF analysis

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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