The amount of NO released following addition of NO donor S-nitrosoglutathione (GSNO) to our experimental buffer (KRH; see below) was measured using an Apollo 1000 Free Radical analyser with an ISO-NOPF100 NO microsensor (1 mm) (World Precision Instruments, USA). The sensor was calibrated with S-nitroso-N-Acetyl-D,L-Penicillamine (Toronto Research) in 0.1 M CuCl2 solution 22 (link). Data were acquired using a Powerlab 2/25 and recorded in LabChart 8.1 (ADInstruments, New Zealand).
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Measuring NO Release with GSNO
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Corresponding Organization :
Other organizations : University of Auckland, University of Otago, University of California, Davis, Johns Hopkins University, University of California, San Diego
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Variable analysis
independent variables
- Amount of S-nitrosoglutathione (GSNO) added to the experimental buffer (KRH)
- Amount of NO released following addition of GSNO
- Experimental buffer (KRH)
- Calibration of Apollo 1000 Free Radical analyser with S-nitroso-N-Acetyl-D,L-Penicillamine (SNAP) in 0.1 M CuCl2 solution
- Positive control: S-nitroso-N-Acetyl-D,L-Penicillamine (SNAP) in 0.1 M CuCl2 solution used for calibration of the NO microsensor
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