Senescence-associated β-galactosidase (SA-β-gal) activity was detected using the cellular senescence assay kit (Chemicon, Temecula, CA), according to the manufacturer’s instructions and as previously described [25 (link)]. In brief, the cells were transfected with anti-miRNA, anti-miR-135b or anti-miR-135a for 48 h and then seeded in seeded in 6-well plates, and incubated for 16 h at 37 °C. Representative microscopic fields were photographed.
Choi S.A., Koh E.J., Kim R.N., Byun J.W., Phi J.H., Yang J., Wang K.C., Park A.K., Hwang D.W., Lee J.Y, & Kim S.K. (2020). Extracellular vesicle-associated miR-135b and -135a regulate stemness in Group 4 medulloblastoma cells by targeting angiomotin-like 2. Cancer Cell International, 20, 558.
Other organizations :
Seoul National University Children's Hospital, Seoul National University, Seoul National University Hospital, Sunchon National University
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