Tumor and Fatty Liver Mouse Models

All protocols for animal experiments were approved by the Ethical Committee on Animal Experiments of Gunma University (18-018), and all animal experiments were conducted under the institutional guidelines. All experiments were performed in compliance with the ARRIVE guidelines. Seven- to nine-week-old Balb/c male mice (CLEA Japan) were used in this study. Tumor transplants were established in 7-week-old male athymic Balb/c nude mice (nu/nu) (CLEA Japan) by subcutaneous injection of a suspension of HCT-116 cells [5 × 10⁶ cells in 150 μL PBS:Matrigel (1:1 v/v)]. Experiments with tumor-bearing mice were performed 2 weeks after the injection of tumor cells. Fatty liver model mice were generated by feeding 6-week-old Balb/c male mice (CLEA Japan) with a choline-deficient, l-amino acid-defined, high-fat diet (CDAHFD, A06071302, Research Diets) consisting of 60 kcal% fat and 0.1% methionine for 2 weeks^{42 (link)}.