Investigating Wing Bud Development in Aphids

To prepare for injection of EcR dsRNA, colonies of A. pisum LSR1 were reared at low density (5–7 aphids per plant) for at least two generations to decrease colony stress and the numbers of alate adults used in the experiments. Then, 45 10-day-old adult aphids were injected with ~0.1 μL of 2 μg/μL EcR or non-targeting E2-Crimson dsRNA. The injected aphids were allowed to recover overnight in petri dishes, then placed onto fava beans with five aphids per plant. Every 24 h the adults were transferred to fresh plants for a total of 3 days (Vellichirammal et al., 2017 (link)). The offspring were then reared to the 4th instar and scored for wing bud development.

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Elston K.M., Maeda G.P., Perreau J, & Barrick J.E. (2023). Addressing the challenges of symbiont-mediated RNAi in aphids. PeerJ, 11, e14961.

Publication 2023

Adults Aphids Dishes Dsrna Fava beans Pisum Plants

Corresponding Organization :

Other organizations : The University of Texas at Austin

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Variable analysis

independent variables

Injection of EcR dsRNA
Injection of non-targeting E2-Crimson dsRNA

dependent variables

Wing bud development in the offspring

control variables

Rearing of colonies at low density (5–7 aphids per plant) for at least two generations to decrease colony stress and the numbers of alate adults used in the experiments
Injection of 10-day-old adult aphids with ~0.1 μL of 2 μg/μL dsRNA
Overnight recovery of injected aphids in petri dishes
Placement of injected aphids onto fava beans with five aphids per plant
Transfer of adult aphids to fresh plants every 24 h for a total of 3 days
Rearing of offspring to the 4th instar for scoring of wing bud development

controls

Positive control: Injection of non-targeting E2-Crimson dsRNA
Negative control: Not mentioned

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