Serial 8-μm sections were cut from paraffin-embedded adult ovaries. All follicles or oocytes in VASA immunostained ovarian sections were counted. Follicle types were classified according to the following structural characteristics. As described previously [67 (link)], the following follicle types were distinguished: primordial follicles (type 1 and type 2), primary follicles (type 3), secondary follicles (type 4 and type 5), and antral follicles (types 6 − 8).
Serial 5-μm sections were obtained from paraffin-embedded ovaries from 5 dpp and newborn mice. The sections were stained with haematoxylin, and the following categories of oocytes and follicles were counted in every fifth section: primordial follicles (oocytes approximately 20 μm in diameter surrounded by 3 − 5 flat pregranulosa cells each); oocytes in cysts (two or more oocytes with shared cytoplasm); and naked oocytes (oocytes without any granulosa cells). As described previously [68 (link)], the sum of these counts was multiplied by five to estimate the total number of oocytes and follicles in each ovary.
Serial 5-μm sections were obtained from paraffin-embedded ovaries from 5 dpp and newborn mice. The sections were stained with haematoxylin, and the following categories of oocytes and follicles were counted in every fifth section: primordial follicles (oocytes approximately 20 μm in diameter surrounded by 3 − 5 flat pregranulosa cells each); oocytes in cysts (two or more oocytes with shared cytoplasm); and naked oocytes (oocytes without any granulosa cells). As described previously [68 (link)], the sum of these counts was multiplied by five to estimate the total number of oocytes and follicles in each ovary.
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