Molecular Regulation of Myoblast Inflammation

C2C12 myoblasts were plated in 6‐well culture dishes at a confluency of 60%. The next day GPR109A gene silencing was obtained by transfection of predesigned siRNA sequences (Cat# AM16708, Life Technologies) using Lipofectamine^® 2000 reagent (Cat# 11668‐027, Life Technologies) according to the manufacturer's instructions. Control cells were transfected with a scrambled siRNA sequence (Cat# AM4642, Life Technologies) as a negative control. At 24 h following transfection, C2C12 were treated with 1 μg/ml lipopolysaccharides (LPS; O111:B4, Sigma‐Aldrich) for 3 h according to published procedures (Frost et al, 2003 (link)). NaB 3 mM (den Besten et al, 2015 (link)) (Cat# 303410 Life Technologies), T0070907 1 μM (Cat# 2301, Tocris UK), MK1903 (Cat# 4622, Tocris UK), and rosiglitazone (Cat# 5325, Tocris UK) were preincubated 1 h before the LPS stimulation.

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Kalkan H., Pagano E., Paris D., Panza E., Cuozzo M., Moriello C., Piscitelli F., Abolghasemi A., Gazzerro E., Silvestri C., Capasso R., Motta A., Russo R., Di Marzo V, & Iannotti F.A. (2023). Targeting gut dysbiosis against inflammation and impaired autophagy in Duchenne muscular dystrophy. EMBO Molecular Medicine, 15(3), e16225.

Publication 2023

Lipofectamine® 2000 reagent LPS; O111:B4 T0070907 MK1903 rosiglitazone

Cells Dishes Lipofectamine 2000 Myoblasts Rosiglitazone Sirna T0070907 Transfection

Corresponding Organization :

Other organizations : National Research Council, Federico II University Hospital, Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Max Delbrück Center, Charité - Universitätsmedizin Berlin, University of Naples Federico II

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