Cytotoxicity of BMAA in IMR-32 and U118-MG Cells

IMR-32 cells were treated with BMAA in a range of different dosages (0, 0.8, 1.6, 3.2, 6.4, 12.8, 25 uM) and incubated under normal growth conditions over a period of 72 hours. U118-MG cells were treated with BMAA in a range of different dosages (0, 1, 5, 25 uM) and incubated under normal growth conditions over a period of 24 hours or seven days. To detect cell lysis, the presence of extracellular lactate dehydrogenase (LDH) was assessed in cell media at 24, 48, and 72 hours using the CyQUANT LDH Cytotoxicity Assay Kit (Invitrogen, Waltham, MA) according to the manufacturer’s instructions. Cell lysis was quantified using a Molecular Devices SpectraMax Microplate Spectrophotometer (San Jose, CA).