Rat CGRP Injection Protocol

Rat CGRP (Sigma-Aldrich; 0.5 nmol) and CGRP_8-37 (Sigma-Aldrich; 0.5 nmol) were diluted in saline. Isoflurane (1.5–2.0%) was used for brief anesthesia during i.c.v. injections. Drug administration was performed by the direct injection into the right lateral ventricle, through the intact scalp, aiming at 1 mm posterior to bregma and 1 mm right from the midline, as described previously^{51 (link)}. H89 (1 mg/kg, Sigma-Aldrich) was administered by intraperitoneal injection, 30 min before CGRP injection.

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Hashikawa-Hobara N., Mishima S., Okujima C., Shitanishi Y, & Hashikawa N. (2021). Npas4 impairs fear memory via phosphorylated HDAC5 induced by CGRP administration in mice. Scientific Reports, 11, 7006.

Publication 2021

Rat CGRP CGRP8-37

Anesthesia Cgrp8 37 Intraperitoneal injection Isoflurane Right ventricle Saline Scalp

Corresponding Organization : Okayama University of Science

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Rat CGRP (Sigma-Aldrich; 0.5 nmol)
CGRP8-37 (Sigma-Aldrich; 0.5 nmol)
H89 (1 mg/kg, Sigma-Aldrich)

dependent variables

Outcomes of the experiments, not explicitly mentioned

control variables

Isoflurane (1.5–2.0%) for brief anesthesia during i.c.v. injections
Direct injection into the right lateral ventricle, through the intact scalp, aiming at 1 mm posterior to bregma and 1 mm right from the midline

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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