Protocol detail

Biofilm Reduction by Peptides and Ciprofloxacin

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To investigate the biofilm-reductive effects of peptides and ciprofloxacin, the biofilm of DRPa-4009 cells was formed on the plastic coverslips for 48 h. After treatments of peptides (64 µM) and ciprofloxacin (256 µM) for 24 h, the coverslips were washed with SP buffer and stained with FilmTracer SYPRO Ruby biofilm matrix stain (Thermo Fisher Scientific Co., Ltd., Seoul, Korea) according to the manufacturer’s protocol. Samples were analyzed by CLSM (A1R HD 25, Nikon, Japan) [30 (link),31 (link)].

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Kim Y.M., Son H., Park S.C., Lee J.K., Jang M.K, & Lee J.R. (2023). Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of Pseudomonas aeruginosa. Antibiotics, 12(2), 349.

Publication 2023

FilmTracer SYPRO Ruby biofilm matrix stain A1R HD 25

After treatments Biofilm Biofilm matrix Buffer Ciprofloxacin Peptides Stain Sypro ruby

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Variable analysis

independent variables

Peptides (64 µM)
Ciprofloxacin (256 µM)

dependent variables

Biofilm of DRPa-4009 cells

control variables

Plastic coverslips
SP buffer
FilmTracer SYPRO Ruby biofilm matrix stain

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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