Western blot analysis was conducted to characterize the expression of BDNF and TrkB, according to previously described methods [12 (link),14 (link)]. Mouse β-actin antibody (1:1,000; Santa Cruz Biotechnology), rabbit BDNF antibody (1:500; Santa Cruz Biotechnology), and rabbit TrkB antibody (1:1,000; Santa Cruz Biotechnology) were used as primary antibodies. The secondary antibodies were horseradish peroxidase conjugated antirabbit antibody for BDNF and TrkB (1:3,000; Vector Laboratories) and horseradish peroxidase-conjugated anti-mouse antibody for β-actin (1:2,000; Vector Laboratories). An enhanced chemiluminescence detection kit (Santa Cruz Biotechnology) was used for band detection.
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Western Blot Analysis of BDNF and TrkB
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Mouse β-actin antibody
rabbit BDNF antibody
rabbit TrkB antibody
horseradish peroxidase conjugated antirabbit antibody for BDNF and TrkB
enhanced chemiluminescence detection kit
Actin
Anti antibody
Antibodies
Antibody
Chemiluminescence
Horseradish peroxidase
Mouse
Rabbit
Trkb
Vector
Western blot analysis
Corresponding Organization :
Other organizations : International St. Mary's Hospital, Catholic Kwandong University, Kyung Hee University, Kyung Hee University Hospital at Gangdong, Kyung Hee University Dental Hospital
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Protocol cited in 2 other protocols
Variable analysis
independent variables
- None explicitly mentioned
- Expression of BDNF
- Expression of TrkB
- Mouse β-actin antibody (1:1,000; Santa Cruz Biotechnology)
- Positive control: Not specified
- Negative control: Not specified
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