Platelet agonists, including ADP, collagen, and thrombin as well as CHRONO-LUME® were purchased from Chrono-log (Havertown, PA). Collagen related peptide (CRP) was a gift from Dr. Peter Newman (Blood Research Institute, WI) as mentioned before.24 (link) Antibodies to phosphorylated AKT (4060S), pan-AKT (2920S), TYMP (4307S), and GST (2624S) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to TYMP (SC-56584), Lyn (SC-7274), and Actin (sc-8432) were purchased from Santa Cruz Biotechnology (Dallas, TX). Antibody to TYMP was also purchased from abcam (ab180783). FITC-conjugated P-selectin antibody (Cat#553744) and the isotype control IgG were purchased from BD Biosciences (San Jose, CA). Tipiracil hydrochloride (TPI) (T2366) was purchased from TargetMol (Boston, MA). All other chemical reagents were purchased from Sigma (St. Louis, MO) except where specifically indicated.
Protocol detail
Platelet Agonists and Signaling Pathways
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Actin
Agonists
Antibodies
Antibody
Blood
Chrono lume
Collagen
Collagen related peptide
Fitc
Isotype
P selectin
Platelet
Thrombin
Tipiracil
Tymp
Corresponding Organization :
Other organizations : Marshall University, Case Western Reserve University
Top 5 similar protocols
Variable analysis
independent variables
- Platelet agonists, including ADP, collagen, and thrombin
- CHRONO-LUME®
- Collagen related peptide (CRP)
- Tipiracil hydrochloride (TPI)
- Phosphorylated AKT
- Pan-AKT
- Actin
- P-selectin
- FITC-conjugated P-selectin antibody (Cat#553744)
- Isotype control IgG
- CHRONO-LUME®
- Isotype control IgG
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