PDLCs were obtained from impacted human wisdom teeth removed at the Department of Dentoalevolar Surgery of the Semmelweis University (Budapest, Hungary) under ethical guidelines approved by the Ethical Committee of the Hungarian Medical Research Council. This study was approved by the Semmelweis University Regional and Institutional Committee of Science and Research Ethics, the number of the ethical permissions are 17458/2012/EKU and 25459/2019/EKU. Isolation of PDLCs was performed according to our previously published protocol [15 (link),81 (link)].
The PDLCs were cultured in Minimal Essential Medium Alpha (αMEM, Gibco, Thermo Fisher, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 2 mM L-glutamine (Gibco), 100 units/mL penicillin and 100 mg/mL streptomycin (Gibco).
The SH-SY5Y human neuroblastoma cell line was purchased from Sigma-Aldrich (Budapest, Hungary) and maintained according to the manufacturer’s protocol. The growth medium of these cells consisted of a 1:1 mixture of Eagle’s MEM and Ham’s F12 medium supplemented with 15% FBS (Gibco), 2 mM L-glutamine (Gibco), 100 units/mL penicillin, and 100 mg/mL streptomycin (Gibco), as well as 1% non-essential amino acids (NEAA, Gibco).
The PDLCs were cultured in Minimal Essential Medium Alpha (αMEM, Gibco, Thermo Fisher, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 2 mM L-glutamine (Gibco), 100 units/mL penicillin and 100 mg/mL streptomycin (Gibco).
The SH-SY5Y human neuroblastoma cell line was purchased from Sigma-Aldrich (Budapest, Hungary) and maintained according to the manufacturer’s protocol. The growth medium of these cells consisted of a 1:1 mixture of Eagle’s MEM and Ham’s F12 medium supplemented with 15% FBS (Gibco), 2 mM L-glutamine (Gibco), 100 units/mL penicillin, and 100 mg/mL streptomycin (Gibco), as well as 1% non-essential amino acids (NEAA, Gibco).
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