Quantifying ZEB1 Expression in Mice Tumors

IHC staining and score calculation were conducted as described previously (20 (link)). Anti-ZEB1 antibody (1:100; cat. no. ab203829; Abcam) was used to detect their expression levels in mouse tumors. Images were visualized using a Nikon ECLIPSE Ti (Nikon Corporation) inverted microscope system (x20 magnification) and processed using Nikon software (CaptureNX2, version 2.4.7).

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Zhao X., Wang D., Ding Y., Zhou J., Liu G, & Ji Z. (2019). lncRNA ZEB1-AS1 promotes migration and metastasis of bladder cancer cells by post-transcriptional activation of ZEB1. International Journal of Molecular Medicine, 44(1), 196-206.

Publication 2019

ab203829 Nikon ECLIPSE Ti CaptureNX2

Anti antibody Microscope Mouse Tumors

Corresponding Organization :

Other organizations : Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, First Hospital of Shanxi Medical University, Shanxi Medical University, The Central Hospital of Shaoyang

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Variable analysis

independent variables

Anti-ZEB1 antibody (1:100; cat. no. ab203829; Abcam)

dependent variables

ZEB1 expression levels in mouse tumors

control variables

Imaging technique (Nikon ECLIPSE Ti inverted microscope system (x20 magnification))
Image processing software (Nikon software, CaptureNX2, version 2.4.7)

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