Cell Proliferation Assay Using xCELLigence

Cell proliferation was assessed using E-16-well plates and the xCELLigence technology (Acea Bioscience, distributed by Roche Diagnostics) as described [51 (link)]. Briefly, cells (2×10³/well) were seeded in 16-well plates in growth medium and left to growth for 72 hrs. Microelectrodes placed on the bottom of plates, detect impedance changes which are proportional to the number of adherent cells and are expressed as Cell Index. The impedance value of each well was automatically monitored by the xCELLigence system and expressed as a Cell Index value. Doubling times for each cell clone were calculated from the cell growth curve during the exponential growth. The experiments were performed at least twice in quadruplicate.