Aneuploidy Detection in Cervical Cells

Cervical cells were lysed using Pharm Lyse lysing buffer (Becton Dickinson, New Jersey, USA) for 30 minutes prior incubation with RNA solution (100 μg/mL) and propidium iodide. DNA fluorescence was measured by flow cytometry (laser excitation at 488 nm and emission above 600 nm), and DNA index was estimated through by comparison the ratio of the DNA content of cells analyzed with labeled blood diploid cells using the ModFitLT V3.0 software (Verity Software House Inc., Topsham, USA). The presence of two peaks on a histogram with a DNA index greater than 1.16 (hyperploidy) or less than 1.00 (hypoploidy), each with more than 10% of the cell population analyzed in the area corresponding to G0–G1 of the cell cycle in the sample, was considered to be aneuploidy [28 (link)].