The method described herein describes the workflow for shotgun lipidomics of lipid extractions from various samples, such as tissue or serum. This purpose of the text is to address the IM-MS workflow, and thus readers are advised to consult the ample literature for appropriate preparation protocols relevant to their sample types [20 (link), 21 (link)]. This text is written for users of the Agilent 6560 with general mass spectrometry knowledge and should provide a novice user with the essential steps towards a successful shotgun lipidomics workflow including preparation of lipid extracts for MS analysis, optimization of instrumental settings, data acquisition, and data analysis of lipids. This work is not intended to be an exhaustive description of related workflows or instrumental parameters.
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Shotgun Lipidomics of Lipid Extracts
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Other organizations : Vanderbilt University
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