Whole-cell lysates of WT, Sun2-/-, and Sun dKO MKCs were prepped as previously described (Stewart et al., 2015 (link)). Primary antibodies against SUN1 (1:100, Abcam, ab124770), SUN2 (1:100; Abcam ab124916), and β-actin (1:1000; mouse; Abcam) were used.
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