Adenovirus Delivery and Bioluminescence Imaging in Mice

8–10-week-old male C57BL/6J mice were purchased from Shanghai Laboratory Animal Center, CAS and were adapted to colony cages with 12 h light/dark cycle in a temperature-controlled environment for 1 week before study. 1 × 10⁹ plaque forming units (pfu) Ad-Aox-luc; 5 × 10⁷ pfu Ad-RSV-β-gal (Rous sarcoma virus promoter); 3 × 10⁸ pfu Ad-GFP, Ad-SIK2, Ad-p300, Ad-p300 S89A, Ad-p300 LXXAA; 1 × 10⁹ pfu Ad-unspecific RNAi (USi), Ad-SIK2 RNAi (SIK2i), Ad-p300 RNAi (p300i) were delivered by tail-vein injection. For in vivo imaging, mice were fasted for 48 h and refed for 2 h and imaged on day 3–5 after adenovirus delivery. Before imaging, mice were injected intraperitoneally with 50 mg/kg Nembutal (Abbott Laboratories) and 100 mg/kg sterile firefly D-luciferin (Biosynth AG). Mice were imaged on the IVIS 100 Imaging System, and analyzed with Living Image software (Xenogen) as described5 (link). All animal studies were approved by the Animal Experiment Committee of Tongji University and in accordance with the guidelines of school of medicine, Tongji University.