In KORA, blood was drawn into S-Monovette® serum gel tubes (SARSTEDT AG & Co., Nümbrecht, Germany) in the morning between 8:00 a.m. and 10:30 a.m. after a fasting period of at least eight hours [17 (link)]. Blood samples were processed according to standard procedures. The samples were stored at −195.79 °C in liquid nitrogen until the execution of metabolic analyses. Details were reported in previous publications [11 (link),17 (link)].
In CARLA, metabolic profiles in the blood serum of the study participants were measured. Blood samples were taken after a supine rest of 30 min. Followed by a 10 min centrifugation (20 °C, 1500 revolutions per min), the samples was collected and deep frozen to −80 °C on the same day until analysis of the metabolites [45 (link)].
In CARLA, metabolic profiles in the blood serum of the study participants were measured. Blood samples were taken after a supine rest of 30 min. Followed by a 10 min centrifugation (20 °C, 1500 revolutions per min), the samples was collected and deep frozen to −80 °C on the same day until analysis of the metabolites [45 (link)].
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