Complete Chloroplast Genome of Forsythia suspensa

Genomic DNA was extracted from fresh young leaves of the F. suspensa plant using the mCTAB method [47 ]. Genomic DNA was fragmented into 400–600 bp using a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Library preparation was conducted using NEBNext^® Ultra™ DNA Library Prep Kit Illumina (New England, Biolabs, Ipswich, MA, USA). Sample sequencing was carried out on an Illumina Hiseq 4000 PE150 platform.
Next, raw sequence reads were assembled into contigs using SPAdes [48 (link)], CLC Genomics Workbench 8 (Available online: http://www.clcbio.com), and SOAPdenovo2 [49 (link)], respectively. Chloroplast genome contigs were selected by BLAST (Available online: http://blast.ncbi.nlm.nih.gov/) [50 (link)] and were assembled by Sequencher 4.10 (Available online: http://genecodes.com/). All reads were mapped to the cp genome using Geneious 8.1 [51 (link)], which verified the selected contigs. The closing of gaps was accomplished by special primer designs, PCR amplification, and Sanger sequencing. Finally, we obtained a high-quality complete F. suspensa cp genome, and the result was submitted to NCBI (Accession Number: MF579702).