Example 7
In the context of gene therapy, inducible gene expression systems may enable gene expression to be triggered at specific times and in specific cell types. To develop a Tet-on system for inducing Klotho gene expression, the ZFP3_VPR construct was cloned into a doxycycline-inducible vector. The ZFP3_VPR and ZFP52_VPR sequences and the inducible vector (Lenti-iCas9-neo, Addgene #85400) were amplified using Clontech HiFi according to the manufacturers protocol and the following primers:
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