Structural Analysis of Heparin by NMR

One-dimensional (1D) ¹H and ¹³C NMR spectra were obtained at 600
MHz on ²H₂O exchanged heparin (20 mg/mL in
²H₂O) using a Bruker Advance II 600 MHz spectrometer (Bruker
BioSpin, Billerica, Massachusetts) with Topspin 3.2 software (Bruker BioSpin) for signal
integration. Two-dimensional HSQC-NMR spectra were obtained at 800 MHz on
²H₂O exchanged heparin (20 mg/mL in ²H₂O)
using a Bruker Advance II 800 MHz spectrometer.^{10 (link)}

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Chen J., Yu Y., Fareed J., Hoppensteadt D., Jeske W., Kouta A., Jin C., Jin Y., Yao Y., Xia K., Zhang F., Chen S., Ye X, & Linhardt R.J. (2019). Comparison of Low-Molecular-Weight Heparins Prepared From Ovine Heparins With Enoxaparin. Clinical and Applied Thrombosis/Hemostasis, 25, 1076029619840701.

Publication 2019

Bruker Advance II 600 MHz spectrometer Topspin 3.2 software

13c nmr Heparin

Corresponding Organization : Rensselaer Polytechnic Institute

Other organizations : Zhejiang University, Loyola University Medical Center

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Variable analysis

independent variables

One-dimensional (1D) 1H and 13C NMR spectra
Two-dimensional HSQC-NMR spectra

dependent variables

Signal integration

control variables

Heparin (20 mg/mL in 2H2O)
Bruker Advance II 600 MHz spectrometer with Topspin 3.2 software
Bruker Advance II 800 MHz spectrometer

controls

Positive control: None mentioned
Negative control: None mentioned

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