TGF-β1 Transgenic Mice Immune Modulation

TGF-β1 Tg+ or TGF-β1 Tg− mice were injected intraperitoneally (i.p.) with neutralizing antibodies raised against CD25 (Li et al., 2008 (link); Liu et al., 2010 (link)) or FGF-9 (Li et al., 2008 (link)). For CD25 neutralizing studies, 125 μg of anti-CD25 (Biolegend # 101906) or IgG2b isotype control (Biolegend #400622) was injected on days 4, 7, and 10 of doxycycline administration and mice were sacrificed on day 14. For FGF-9 blocking experiments, 250 μg of anti-FGF-9 (R&D #MAB273) or IgG2A isotype control (R&D #MAB003) were injected on days—1, 2, 5, 8, and 11 of doxycycline administration and mice were sacrificed on day 14.

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Peng X., Moore M.W., Peng H., Sun H., Gan Y., Homer R.J, & Herzog E.L. (2014). CD4+CD25+FoxP3+ Regulatory Tregs inhibit fibrocyte recruitment and fibrosis via suppression of FGF-9 production in the TGF-β1 exposed murine lung. Frontiers in Pharmacology, 5, 80.

Publication 2014

MAB003

Cd25 Doxycycline Fgf 9 Igg2a Igg2b Isotype Mice Neutralizing antibodies Tgf β1

Corresponding Organization :

Other organizations : Yale University, Second Xiangya Hospital of Central South University, Central South University

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Variable analysis

independent variables

Injection of neutralizing antibodies against CD25 or FGF-9

dependent variables

Not explicitly mentioned

control variables

Injection of IgG2b isotype control (for CD25 neutralizing studies)
Injection of IgG2A isotype control (for FGF-9 blocking experiments)

controls

Positive control: Injection of neutralizing antibodies against CD25 or FGF-9
Negative control: Injection of IgG2b or IgG2A isotype control

Annotations

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