Naphthalene (Sigma Adrich #84679) was dissolved in corn oil at a concentration of 20 mg/mL at room temperature and sterile filtered before administration. C57Bl/6J or CCSP-CreER+/−; R26-LSL-YFP/iDTR mice were injected intraperitoneally with Naphthalene at 200 mg/kg body weight or an equivalent volume of corn oil for control animals. Mice were then monitored for signs of distress throughout recovery until sacrificed with anesthetic overdose at indicated timepoints.
Protocol detail
Naphthalene Lung Injury Model
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Corresponding Organization : Harvard Stem Cell Institute
Other organizations : Harvard University Press, Harvard College Observatory, University College London
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Variable analysis
independent variables
- Naphthalene concentration (20 mg/mL)
- Naphthalene dose (200 mg/kg body weight)
- Signs of distress during recovery
- Timepoints of sacrifice
- Corn oil administration for control animals
- Mouse strains (C57Bl/6J or CCSP-CreER+/−; R26-LSL-YFP/iDTR)
- Negative control: Corn oil administration for control animals
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