Immunofluorescent Localization of TSEN54 in Cerebrum

Immunofluorescence was performed to visualize the distribution and localization of TSEN54 in the cerebrum according to an established protocol [39 (link)]. For this purpose, a monoclonal mouse anti-human-TSEN54-antibody (anti-TSEN54, sc-398327, dilution 1:50, Santa Cruz Biotechnology, Inc) was used. Following the same procedure as described above, after dewaxing and antigen retrieval, non-specific binding was blocked with 20% goat serum diluted in diluted in PBS/0.1% Triton X/1% BSA. Thereafter the sections were incubated with the primary anti-TSEN54 antibody, diluted in PBS/0.1% Triton X/1% BSA. Sections for negative control were incubated with non-immune serum.
Goat-anti-mouse IgG CyTM3 (115-165-166, dilution 1:200, Jackson ImmunoResearch) was used as secondary antibody. Nuclear counter-staining was performed with 0.01% bisbenzimide (Sigma-Aldrich Chemie GmbH) and sections were mounted with Dako fluorescence mounting medium (DakoCytomation GmbH).