Cell concentrations was measured using Hemocytometer Standard Specification (Improved Neubauer) (HIRSCHMANN LAB, Baden-Württemberg, Germany). 3 ml of PBS (D-PBS(-) without Ca and Mg, #14249–95, Nacalai Tesque, Kyoto, Japan) containing 0.25–1.0×104 cells were added onto a dish (PrimeSurface Dish 35, #MS-9035X, Sumitomo Bakelite, Tokyo, Japan).
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