Liver pathology was evaluated using conventional histological analysis on hematoxylin and eosin (H & E) stained sections. Further evaluation of pathology was achieved by high-resolution light microscopy using glutaraldehyde-fixed, osmium tetroxide post-fixed, epoxy-embedded, and toluidine blue-stained sections (11 (link), 13 (link)) and electron microscopy. Liver fibrosis was evaluated by Sirius red staining of formalin-fixed, paraffin-embedded liver sections for visualization of collagen. Accumulation of intrahepatic fat and collagen content was quantified by computerized densitometry using the ImagePro Plus software (11 (link)–13 (link)). Further evaluation of liver fibrosis was assessed by measuring transforming growth factor alpha (TGF- α) and beta (TGF- β) (18 (link), 19 (link)) by quantitative RT-PCR. For electron microscopic studies, thin sections from selected tissue blocks were sectioned with an LKB ultramichrotome, stained with uranyl acetate, and examined with a Hitachi 600 electron microscope (Hitachi, Indianapolis, USA), as described before (11 (link), 13 (link)).
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Comprehensive Liver Pathology Assessment
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Collagen
Densitometry
Embedded paraffin
Eosin
Epoxy
Fibrosis of liver
Formalin
Glutaraldehyde
Light microscopy
Liver
Microscope electron
Osmium tetroxide
Rt pcr
Tgf α
Tgf β
Thin sections
Tissue
Toluidine blue
Uranyl acetate
Corresponding Organization : Charles R. Drew University of Medicine and Science
Other organizations : University of California, Los Angeles
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Variable analysis
independent variables
- Liver pathology evaluation method: conventional histological analysis on hematoxylin and eosin (H & E) stained sections, high-resolution light microscopy using glutaraldehyde-fixed, osmium tetroxide post-fixed, epoxy-embedded, and toluidine blue-stained sections, and electron microscopy
- Liver fibrosis
- Accumulation of intrahepatic fat
- Collagen content
- Transforming growth factor alpha (TGF-α) and beta (TGF-β) expression
- None explicitly mentioned
- None explicitly mentioned
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