PCNA expression analysis in tumor tissues

Tumor tissues were fixed with 4% paraformaldehyde (Solarbio) and encapsulated in paraffin. IHC analysis was used to analyze PCNA expression as reported [30 (link)]. The sections (4 µm) of the tumors were hatched with diluted anti-PCNA antibody (ab29, 1:1000, Abcam) and biotinylated IgG secondary antibody (ab64256, 1:500 dilution, Abcam), stained with 3,3’-diaminobenzidine substrate (Invitrogen), and observed under microscopy (Leica TCS SP5) and photographed.

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Cui X., Zhang B., Li B, & Li X. (2022). Circular RNA circ_0002360 regulates the Taxol resistance and malignant behaviors of Taxol-resistant non-small cell lung cancer cells by microRNA-585-3p-dependent modulation of G protein regulated inducer of neurite outgrowth 1. Bioengineered, 13(4), 9070-9085.

Publication 2022

4% paraformaldehyde ab64256 3,3’-diaminobenzidine substrate TCS SP5

Anti antibody Dilution Igg antibody Microscopy Paraffin Paraformaldehyde Pcna Tissues Tumors

Corresponding Organization :

Other organizations : First Affiliated Hospital of Xi'an Jiaotong University, Weinan Central Hospital

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Variable analysis

independent variables

Tumor tissues were fixed with 4% paraformaldehyde (Solarbio) and encapsulated in paraffin.

dependent variables

PCNA expression

control variables

Sections (4 µm) of the tumors were prepared

controls

No positive or negative controls were explicitly mentioned.

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