Transgenic Mouse Models for Neural Circuit Analysis

These experiments used adult (4–6 months old) male C57BL/6J wild-type mice (Charles River Laboratories, UK) and transgenic mice heterozygous for the transgene expressing the Cre recombinase under the control of either the vesicular-glutamate transporter-2 (Vglut2) or the dopamine transporter (DAT) promoter (Jackson Laboratories; obtained from C57BL/6J crossed with Vglut2-ires-cre B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ, stock number 028863, RRID: IMSR_JAX:028863; or with DAT-ires-cre B6.SJL-Slc6a3tm1.1(cre)Bkmn/J, stock number 006660, RRID: IMSR_JAX:006660) or for the transgene expressing the optimized Flp recombinase under the control of the DAT promoter (Jackson Laboratories; obtained from C57BL/6J crossed with DAT-P2A-Flpo Slc6a3em1(flpo)Hbat/J, stock number 035436, RRID: IMSR_JAX:035436)^{120 (link)–122 (link)}. Animals were housed with their littermates up until the start of the experiment. All mice held in IVCs, with wooden chew stick, nestlets and free access to water and food ad libitum in a dedicated housing facility with a 12/12 h light/dark cycle (lights on at 07:00), 19–23°C ambient temperature and 40–70% humidity. Experimental procedures performed on mice in accordance with the Animals (Scientific Procedures) Act, 1986 (United Kingdom), with final ethical review by the Animals in Science Regulation Unit of the UK Home Office.

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Clarke-Williams C.J., Lopes-dos-Santos V., Lefèvre L., Brizee D., Causse A.A., Rothaermel R., Hartwich K., Perestenko P.V., Toth R., McNamara C.G., Sharott A, & Dupret D. (2024). Coordinating brain-distributed network activities in memory resistant to extinction. Cell, 187(2), 409-427.e19.

Publication 2023

C57BL/6J wild-type mice Vglut2 C57BL/6J

Corresponding Organization : University of Oxford

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Variable analysis

independent variables

Transgenic mouse genotype
Promoter driving Cre or Flp recombinase expression (Vglut2, DAT)

dependent variables

Not explicitly mentioned

control variables

Age of mice (4-6 months old)
Sex of mice (male)
Mouse strain (C57BL/6J wild-type)
Housing conditions (IVCs, wood chew stick, nestlets, ad libitum food and water, 12/12 h light/dark cycle, 19-23°C, 40-70% humidity)
Experimental procedures (performed in accordance with the Animals (Scientific Procedures) Act, 1986 (United Kingdom))

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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