CXCR4 Receptor Internalization Assay
Corresponding Organization : Inserm
Other organizations : Université de Bretagne Occidentale, Cornell University
Variable analysis
- Dex (125 nM)
- SDF-1α (100 nM)
- CXCR4 receptor internalization
- Ca2+ signaling
- Cells maintained in RPMI medium
- Incubation temperature (4 °C and 37 °C)
- Staining FACS buffer (PBS, 0.5% BSA, 0.05% NaN3, and 5% FBS)
- Anti-human CXCR4-APC antibody (5:200 dilution, clone 12G5, eBioscience)
- Isotype control APC-Mousse IgG 1k antibody (1:200 dilution, clone MOPC-21, Biolegend and BD Biosciences)
- Unstimulated (medium alone) cells processed in parallel
- Isotype control APC-Mousse IgG 1k antibody (1:200 dilution, clone MOPC-21, Biolegend and BD Biosciences)
Annotations
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