Western Blot Analysis of Protein Expression

Western blot analyses were performed as described previously 6 (link), 15 (link). Total protein of LoVo and HCT116 was extracted before and after treatment and the concentration was determined. The total protein was separated on sodium dodecyl sulfate polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Amersham Hybond-P PVDF Membrane; GE Healthcare). The primary antibody used was CK7, with β-actin (Sigma-Aldrich) used as a control.

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Fei F., Li C., Cao Y., Liu K., Du J., Gu Y., Wang X., Li Y, & Zhang S. (2019). CK7 expression associates with the location, differentiation, lymph node metastasis, and the Dukes' stage of primary colorectal cancers. Journal of Cancer, 10(11), 2510-2519.

Publication 2019

Amersham Hybond-P PVDF Membrane β-actin

Actin After treatment Antibody Membrane Polyacrylamide gels Protein Pvdf Sodium dodecyl sulfate Western blot analyses

Corresponding Organization :

Other organizations : Nankai University, Tianjin Medical University, Tianjin University of Traditional Chinese Medicine, Logistics University of People's Armed Police Force, Tianjin People's Hospital

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Variable analysis

independent variables

Treatment of LoVo and HCT116 cells

dependent variables

Total protein expression of CK7

control variables

β-actin protein expression

controls

Positive control: β-actin
Negative control: Not explicitly mentioned

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