B16-mHEL-GFP mouse melanoma cells [13 (link)] and 40LB [11 (link)] were maintained in D-MEM medium (high glucose; Wako) supplemented with 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin (GIBCO) in a humidified atmosphere at 37°C with 5% CO2.
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