The MK/T-1 murine corneal fibroblast cell line was maintained in DMEM-low glucose media containing 10% FBS and 50 μg/ml hygromycin (Invitrogen) at 37°C as described (20 (link), 21 (link)), and cells were harvested when 70% confluent. Bone marrow derived macrophages were obtained from naïve C57BL/6 mice as described (12 (link)). Briefly, total bone marrow cells were collected from the femurs and tibias of mice, incubated in erythrocyte lysis buffer (eBioscience), and cultured in a 6ml bacteriologic-grade Petri dish containing Macrophage Growth Media (DMEM with L-glutamine, Na-pyruvate, HEPES, penicillin/streptomycin, 10% FBS, and 30% L929 cell-conditioned media) at 37°C. Growth medium was replaced on day 5 and adherent cells were isolated on day 7. MK/T-1 cells and macrophages were then cultured with media alone, or media containing100 μg/ml Aspergillus hyphal extract, 10ng/ml of recombinant mouse IL-17A (R&D), or both for three hours at 37°C. Supernatants were collected and chemokines were analyzed by ELISA.