Postnatal Dorsal SVZ Electroporation

Postnatal brain electroporation (Boutin et al., 2008 (link)) was adapted to target the dorsal SVZ. Briefly, postnatal day 1 (P1) pups were cryoanesthetized for 2 min on ice and 1.5 μl of plasmid was injected into their left ventricle using a glass capillary. Plasmids were injected at a concentration of 2–2.5 μg/μl. Electrodes (Nepagene CUY650P10) coated with highly conductive gel (Signagel, signa250) were positioned in the dorsoventral axis with the positive pole dorsal. Five electric pulses of 100 V, 50 ms pulse ON, 850 ms pulse OFF were applied using a Nepagene CUY21-SC electroporator. Pups were immediately warmed up in a heating chamber and brought to their cages at the end of the experiment.

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Merour E., Hmidan H., Marie C., Helou P.H., Lu H., Potel A., Hure J.B., Clavairoly A., Shih Y.P., Goudarzi S., Dussaud S., Ravassard P., Hafizi S., Lo S.H., Hassan B.A, & Parras C. (2022). Transient regulation of focal adhesion via Tensin3 is required for nascent oligodendrocyte differentiation. eLife, 11, e80273.

Publication 2022

CUY21-SC electroporator

Axis Brain Capillary Conductive Electric Electroporation Left ventricle Plasmids Pulse Pulses

Corresponding Organization :

Other organizations : Institut du Cerveau, Inserm, Sorbonne Université, Centre National de la Recherche Scientifique, University of California, Davis, University of Portsmouth

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Variable analysis

independent variables

Plasmid concentration (2-2.5 μg/μl)
Electric pulse parameters (100 V, 50 ms pulse ON, 850 ms pulse OFF, 5 pulses)

dependent variables

Not explicitly mentioned

control variables

Postnatal day 1 (P1) pups
Cryoanesthesia for 2 min on ice
Injection volume (1.5 μl)
Injection site (left ventricle)
Electrode positioning (dorsoventral axis with positive pole dorsal)
Warming up in a heating chamber after electroporation

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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