Genomic DNA Extraction and Sequencing

The GENTRA Blood kit (Qiagen N.V.) was used for the isolation of genomic DNA from EDTA blood samples. The quantification and quality of the obtained DNA were assessed using the Qubit fluorometer (Qiagen N.V.). DNA was paired-end sequenced (read length of 150 base pair) as single-indexed genomic libraries using the Illumina Novaseq6000 (Illumina Inc., USA). Finally, raw reads were preprocessed by trimming the adapter sequences and removing the reads with 50% of low-quality nucleotides and fewer than 36 base pairs in length with fastp v0.23.1 [26 (link)].

Free full text: Click here

Gonzalez-Prendes R., Ginja C., Kantanen J., Ghanem N., Kugonza D.R., Makgahlela M.L., Groenen M.A, & Crooijmans R.P. (2022). Integrative QTL mapping and selection signatures in Groningen White Headed cattle inferred from whole-genome sequences. PLOS ONE, 17(10), e0276309.

Publication 2022

GENTRA Blood kit Qubit fluorometer Novaseq6000

Blood Edta Genomic Genomic libraries Isolation Nucleotides

Corresponding Organization : Animal Production Institute

Top 5 similar protocols

Variable analysis

independent variables

Isolated genomic DNA from EDTA blood samples using the GENTRA Blood kit (Qiagen N.V.)

dependent variables

Quantification and quality of the obtained DNA using the Qubit fluorometer (Qiagen N.V.)
DNA paired-end sequenced (read length of 150 base pair) as single-indexed genomic libraries using the Illumina Novaseq6000 (Illumina Inc., USA)

control variables

Preprocessed raw reads by trimming the adapter sequences and removing the reads with 50% of low-quality nucleotides and fewer than 36 base pairs in length with fastp v0.23.1

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!