The bending rigidity of E. coli cells was measured using a microfluidic assay similar to that used in Refs. 20 (link),21 (link). E. coli MG1655 was transformed with a plasmid (pDB192) containing sulA under an isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter. Cells were grown overnight in 2 mL LB containing 30 μg/mL kanamycin and 50 μg/mL ampicillin. IPTG was added to the medium to induce sulA throughout cell growth in the microfluidic flow chamber. Deflection of cells under fluid flow was monitored on a Zeiss Axiovert 100 microscope (Zeiss) equipped with a 60X oil objective. Images were collected with an Andor iXon 3 EMCCD (Andor) using μManager v. 1.426 . Deflection of the cells was determined using a custom Igor Pro (WaveMetrics Inc.) image-analysis algorithm.
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Microfluidic Bending Rigidity of E. coli
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Other organizations : Stanford University, University of Wisconsin–Madison, University of California, San Francisco
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Variable analysis
independent variables
- Induction of sulA expression using IPTG
- Bending rigidity of E. coli cells
- E. coli MG1655 strain
- Plasmid (pDB192) containing sulA under an IPTG-inducible promoter
- Growth medium (LB) with kanamycin and ampicillin
- Microfluidic flow chamber
- Microscope and image analysis setup
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