Bilateral adrenalectomy was performed in wild-type adult female mice. The adrenalectomized animals were supplied with 0.9% saline to maintain salt levels. One week after surgery, the AAV virus (AAV5-saCas9-Fshr) was stereotaxically injected into the pituitary to knock down the expression of Fshr. Mice were humanely euthanized if postsurgical complications progressed to a pre-defined humane endpoint at which the mice started to suffer.
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