The CD of Circular Dichroism analyzer (J-815, JASCO, Japan) was used to monitor the changes of PolyCHb secondary structure before and after AA addition. Add 30 μL PolyCHb sample solution to 3 mL 0.05 mol/L PBS solution with pH 7.5 in the cuvette for CD analysis, and then obtain CD spectrum (190–250 nm).
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