CyTOF Antibody Profiling Protocol

Table S1 contains all the 38 metal-conjugated antibodies with specifications used in CyTOF experiments. EQ beads (Fluidigm) were used as a loading control. All data were produced on a Helio3 CyTOF Mass Cytometer (Fluidigm). Mass cytometry data files were normalized using the bead-based Normalizer (13 (link)). The output FCS files were randomized and homogenized with the EQTM Four Element Calibration beads against the entire run, per the manufacturer’s recommendations (14 (link)). CyTOF analysis was performed by PLTTech Inc. (Hangzhou, China) as described previously (15 (link)).

Free full text: Click here

Jin J., Wang L., Liu Y., He W., Zheng D., Ni Y, & He Q. (2023). Depiction of immune heterogeneity of peripheral blood from patients with type II diabetic nephropathy based on mass cytometry. Frontiers in Endocrinology, 13, 1018608.

Publication 2022

EQ beads Helio3 CyTOF Mass Cytometer

Antibodies Metal

Corresponding Organization : Zhejiang Chinese Medical University

Other organizations : Hangzhou Medical College, Zhejiang Provincial People's Hospital, Huazhong University of Science and Technology, Central Hospital of Wuhan

Top 5 similar protocols

Variable analysis

independent variables

Metal-conjugated antibodies

dependent variables

Mass cytometry data

control variables

EQ beads (Fluidigm) used as loading control
EQTM Four Element Calibration beads used for data normalization

controls

Positive control: EQ beads (Fluidigm) used as loading control
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!